工作经历

2014.01- 华南农业大学生命科学学院 教授

2010.06-2013.12 伯明翰大学生物系 Research Fellow 

2009.11-2010.05 伯明翰大学生物系 Research Assistant 

2002.08-2005.10 中国科学院武汉病毒研究所 助理研究员 

学习经历
学位学历学科门类专业毕业学校类型入学时间毕业时间
博士博士研究生
理学
细胞生物学
伯明翰大学
全日制 2005-102010-01
硕士硕士研究生
理学
生物化学与分子生物学
中国科学院武汉病毒研究所
全日制 1999-082002-07
学士大学本科
理学
生物技术
湖北大学
全日制 1995-091999-07
研究兴趣

1.动物病原与宿主细胞的相互作用

针对结核杆菌以及禽流感等重要的人畜共患病病原,开展宿主的免疫细胞对病原的免疫应答研究,在细胞生物学水平和分子生物学水平上,揭示人畜共患病病原和宿主免疫细胞内的mRNP以及蛋白质相互作用关系。

2. 真核生物细胞RNA对细胞的重要生物过程的影响

    以哺乳动物和酵母为主要研究对象,揭示RNA降解机理的保守性和特异性。在免疫细胞内主要开展:相关的病原外源性蛋白进入免疫细胞后,RNA分子的多样性分析、RNA剪接机理的变化以及相关RNA的稳定性变化的研究。在酵母的研究中主要开展验证性研究,揭示其保守性和特异性。
发表文章

K.-Jubran1, J. Wen1, S. R.Chaudhury, M. Li, A. Abdullahi, P. Ramanathan, K. Piechoki, K. N. Rugjee and S.Brogna . (2013) Visualization of the Assembly of 80S Ribosomes Reveals thePresence of Functional Ribosomes in the Nucleus. RNA 19(12) 1669-83.

K. N.Rugjee, S. R. Chaudhury, K. A. Jubran, P. Ramanathan, T. Matina, J. Wen and S. Brogna (2013). Fluorescentprotein tagging confirms the presence of ribosomal proteins at Drosophilapolytene chromosomes. PeerJ 1:e15; DOI 10.7717/peerj.15

Li Z, Wen J, Lin Y, Wang S, Xue P, etal. (2011) A Sir2-Like Protein Participates in Mycobacterial NHEJ. PLoS ONE6(5): e20045. 

Wen, J., andBrogna. S. (2010) Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe. EMBO Journal 29(9) 1537-1551.  

Brogna,S., and Wen, J. (2009). Nonsense-mediated mRNA decay(NMD) mechanisms (Perspective). NatStruct Mol Biol 16, 107-13.

Bonander,N., Darby, R.A., Grgic, L., Bora, N., Wen,J., Brogna, S., Poyner, D.R., O'Neill, M.A., and Bill, R.M. (2009).Altering the ribosomal subunit ratio in yeast maximizes recombinant proteinyield. Microb Cell Fact 8, 10.

Li, F.,Zhang, Z., Peng, J., Cui, Z., Pang, D., Li, K., Wei, H., Zhou, Y., Wen, J., and Zhang, X. (2009). Imaging viralbehavior in Mammalian cells with self-assembled capsid-quantum-dot hybridparticles. Small 5, 718-26.

Li, N.,Cui, Z., Wen, J., Zhang, Z., Wei, H., Zhou, Y., andZhang, X. (2008). Live cell imaging of protein interactions in poliovirus RNAreplication complex using fluorescence resonance energy transfer (FRET).BiochemBiophys Res Commun 368,489-94. 

Brogna,S., Ramanathan, P., and Wen,J. (2008). UPF1 P-bodylocalization. Biochem SocTrans 36, 698-700.

Wen, J., andBrogna, S. (2008). Nonsense-mediated mRNA decay. Biochem Soc Trans 36, 514-6. 

Guo, Y.,Zhou, Y., Zhang, X., Zhang, Z., Qiao, Y., Bi, L., Wen, J., Liang, M., and Zhang, J. (2006). Phagedisplay mediated immuno-PCR. NucleicAcids Res 34, e62.

Zhang,J., Zhang, X., Zhou, Y., Bi, L., Zhang, Z., Wang, S., Chen, Y., Guo, Y., Wen, J., and Yu, Z. (2007), Construction andCharacterization of an Anti-Prion scFv Fusion Protein Pair for Detection ofPrion Protein on Antibody Chip, AnalyticalLetters, 40:5, 855 – 873.

You, F.,Zhou, Y., Zhang, X., Huang, Z., Bi, L., Zhang, Z., Wen, J., Chen, Y., Jiang, G., and Zheng, M.(2006). Cell-free bioassay for measurement of dioxins based on fluorescenceenhancement of fluorescein isothiocyanate-labeled DNA probe. Anal Chem 78, 7138-44. 

Cui, Z.,Zhang, Z., Zhang, X., Wen, J.,Zhou, Y., and Xie, W. (2005). Visualizing the dynamic behavior of poliovirusplus-strand RNA in living host cells. NucleicAcids Res 33, 3245-52. 

Wen, J., Zhang,X., Cheng, Z., Liu, H., Zhou, Y., Zhang, Z., Yang, J., and Deng, J. (2004). Avisual DNA chip for simultaneous detection of hepatitis B virus, hepatitis Cvirus and human immunodeficiency virus type-1. Biosens Bioelectron 19, 685-92. 

Wang, S., Wen, J., Zhou, Y., Zhang, Z., Yang, R., Zhang,J., Chen, J., and Zhang, X. (2004). Identification and characterization ofBacillus anthracis by multiplex PCR on DNA chip. Biosens Bioelectron 20, 807-13. 

Wen, J., Zhang, X., Zhou, Y., Wang, J., Zhang,Z. and Zhang, X. (2003). A Genotype 3, Subtype 3b of Hepatitis C Virus Found inWuhanby Using Gene Chip. ChineseJournal of Virology 19,274-7. (In Chinese, English Abstract available)

一种基因芯片上的酶标信号检测方法,专利号:ZL 02 115833.9,发明人:张先恩,邓教宇,黄旭,张治平,邵文海,文继开,陆红兵,刘强。

参加的国际会议


TERM meeting, Gif sur Yvette. 口头报告, May 2010, May 2011 and Mar2012.
British Yeast Group meeting, Brighton. --口头报告,Mar 2011.
RNA Splicing, Rydal. --口头报告, Feb 2011.
British Yeast Group meeting, Oxford. --口头报告, Mar 2010.
EMBO Protein Synthesis andTranslational Control, Heidelberg. 墙报, Sep 2009.
Gene Expression and Analysis, Manchester. --口头报告, Mar 2008.
RNA UK meeting, Lake District. --口头报告, Jan2008.
RNA Conference, Berlin, Germany. --口头报告, Aug 2008.
Eighth World Congress on Biosensors, Granada, Spain. 墙报, May, 2004.