工作经历

2009.07-至今

      华南农业大学生命科学学院 教授

 

2007.07-2009.06

      哥伦比亚大学医学院辐射研究中心 (Center for Radiological Research, Columbia University Medical Center, New York, NY, USA)  博士后研究科学家

 

2006.12-2007.06

      劳伦斯伯克莱国家实验室生物学分部 (Life science division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA)  访问博士后学者

 

2003.12-2006.12  

      罗格斯大学药学院药学系 (Department of Pharmaceutics, College of Pharmacy, Rutgers, The State University of New Jersey, New Jersey, USA) 博士后/助理研究员/副研究员

学习经历
学位学历学科门类专业毕业学校类型入学时间毕业时间
博士博士研究生
哲学
Plant Molecular biology
罗格斯大学(Rutgers University)
全日制 1998-092003-12
学士大学本科
理学
化学系
中山大学
全日制 1992-091996-07
研究兴趣

药物转运蛋白的结构-功能关系

药物转运蛋白在兽药吸收、分布和排除(ADME)中的作用及其调控

膜蛋白在对外界环境改变的反应中的信号传导作用

讲授课程

本科生授课:

  动物生物化学 (双语授课)

  生物化学A(双语授课)

  生物化学C

  生化与分子生物学专题

  生物技术进展

  生物专业英语

研究生授课: 

  生物化学与分子生物学专业英语

  生物化学与分子生物学文献综述

  生化与分子生物学进展

实验室研究项目

1.人体转运蛋白的结构-功能关系研究:

      药物转运蛋白和药物代谢酶,药物目标的共同作用决定了人体对许多药物的反应。因此,药物转运是生物体对药物吸收的关键环节。转运蛋白功能的缺失,会造成人体药物排除或解毒障碍,从而造成药物诱导的肝或肾衰竭。近年来,由于认识到转运蛋白在药物转运中的重要作用,许多大型跨国药厂已把对这类蛋白在药物动力学和药效学上的研究作为药物研发中的重要一环。然而,迄今为止,对它们的结构-功能关系的研究只有不多的报道。本实验室利用各种生化技术,开展对药物转运蛋白结构-功能关系的系统研究,鉴定其序列中起关键作用的氨基酸,探索它们翻译后调节控制机理。

 

2.药物转运蛋白在兽药吸收和排除中的作用:

      随着畜牧业的现代化、集约化和规模化生产,兽药及兽药添加剂在降低发病率与死亡率、提高饲料利用率、促进生长和改善肉品品质方面起着十分显著的作用,已成为现代畜牧业发展不可缺少的物质基础。但是,由于饲养技术的落后和经济利益的驱使,养殖过程中滥用兽药和超标使用兽药的现象普遍存在。各种药物及激素残留对于人体健康和生态环境所带来的不良影响日益引起关注。对药物的吸收效率,是由吸收转运蛋白和外排转运蛋白协同作用而共同决定的。通过系统研究转运蛋白在食品动物药物吸收和排泄中的作用,将能填补吸收转运蛋白在兽药生物利用度中所起的作用方面的空白,为有效地提高兽药的吸收分布提供重要的理论依据。

 

3. 膜蛋白在信号传导中的作用

      膜蛋白是否能正确地表达在特定的膜结构中是其发挥正常功能的关键因素,膜蛋白的翻译后修饰,在细胞器如内质网、高尔基体中的处理过程受多种因素的影响。细胞通过调控膜蛋白在细胞膜表面的表达量和表达位点,对各种外界环境变化(如营养元素量的改变、外界刺激等)作出反应。实验室的另一研究方向是以生物化学和细胞生物学方法,研究膜蛋白在信号传导过程的重要作用,探索其所介导的细胞对外界刺激如辐射、环境毒物的反应。

发表论文

专著章节:Hong, M., You, G. (2007) Regulation of drug transporter activity (chapter 17, pp517-533) in Drug Transporters: Molecular Characterization and Role in Drug Disposition. Eds Guofeng You, Marilyn Morris and Binghe Wang. John Wiley & Son Inc

论文: 1.     

1. ZhouC, Rong Y,Konishi T,Xiang Z,Zihui F,Hong M2017Effect of Carbon-IonRadiation on Drug Transporters Organic Anion Transporting Polypeptides inBreast Cancer Cells. Radiat Res. 187(6):689-700. 2017 Apr 7. [Epub ahead of print] (IF:2.539, JCR: 三区)

2. Ni C, Yu X, Fang Z, Huang J, Hong M. (2017) Oligomerizationstudy of human organic anion transporting polypeptide 1B1.Mol Pharmceutic. 14(2):359-367. 2016 Dec 9 (IF: 4.440, 5yr IF: 4.961, JCR: 二区)

3.  HongM (2017) Biochemical studies on thestructure-function relationship of major drug transporters in the ATP-bindingcassette family and solute carrier family. Adv Drug Deliv Rev. 116(2017): 3-20. 2016 Jun 15.  (IF:11.764, 5yr IF: 17.214, JCR:一区)

4. Fang Z, Xu A, Wu L, Hei TK, HongM (通讯作者)(2016) The role of protein kinase C alpha translocation in radiation-induced bystander effect. Sci Rep. 2016 May 11;6:25817.IF:5.228, 5yr IF: 5.525, JCR: 二区)

5.  HongW, WuZ, FangZ, HuangJ, HuangH, Hong M (通讯作者)2015Amino acid residues in the putative transmembrane domain11 of human organic anion transporting polypeptide 1B1 dictate transporter substratebinding, stability, and trafficking. Mol Pharmaceut. 2015 Dec 7;12(12):4270-6. 2015 Nov 20. [Epub ahead of print] (IF: 4.342, 5yr IF:4.961, JCR: 二区)
6.  Hong M (通讯作者), HongW, Ni C, Huang J, Zhou C.(2015) Protein kinase C affects the internalizationand recycling of organic anion transporting polypeptide 1B1. BiochimBiophys Acta. 1848(10 Pt A):2022-2030.2015 May 22. (IF: 3.687, 5yr IF:3.881, JCR: 三区)
7.  HuW, XuS, YaoB, Hong M, WuX, PeiH, ChangL, DingN, GaoX, YeC, WangJ, HeiTK, Zhou G.2014MiR-663 inhibits radiation-induced bystander effectsby targeting TGFB1 in a feedback mode. RNA Biol. 11(9):1189-98
8.  Xiao Y,Deng J, Liu X, Huang J, Sun Y, Dai R, Hong M (通讯作者) (2014) Different binding sites of bovine organic anion-transportingpolypeptide1a2 are involved in the transport of different fluoroquinolones. Drug Metab Dispos. 42(8):1261-7 (IF: 3.2525yrIF: 3.614, JCR: 三区)
9.  Hong M. (2014) Heavy ion-related bystander effects. Rend.Fis. Acc. Lincei 25(1 supp): 65-68. ( IF: 0.75, 5yr IF: 0.85, JCR: 四区)
10.  HongM.2014Critical domains within the sequence of human organicanion transporting polypeptides. Curr Drug Metab. 15(3):265-70. 2013 Dec 28. [Epub ahead of print] (IF: 2.9765yrIF: 4.205, JCR: 2)
11.  HuangJ, Li N, Hong W, Zhan K, Yu X,Huang H, Hong M. (通讯作者)(2013)Conserved tryptophan residues within putative transmembrane domain 6 affect transportfunction of organic anion transporting polypeptide 1B1.Mol Pharmacol. 84(4):521-7. (IF: 4.125yrIF: 4.596, JCR: 2)
12.  Zhan K, Yao J, Huang J, Zhou G, Li W, Chen L, Hong M (通讯作者)(2013)Effect of X-ray irradiation on expression of organic anion transportingpolypeptides. Int J Radiat Biol. 89(10):832-40 (IF: 1.8375yr IF: 2.009, JCR: 医学4区,核医学3区,核科学技术1)
13.  Liu, X, Huang, J, Sun, Y, Zhan, K, Zhang, Z, Hong, M (通讯作者)(2013) Identification of multiplebinding sites for substrate transport in bovine organic anion transporting polypeptide1a2. Drug Metab Dispos, 41(3):602-607, Epub 2012 Dec 19. (IF: 3.3345yr IF: 3.827, JCR: 三区)
14.  Yu, Y,Liu, X, Zhang, Z, Xiao, Y, Hong, M (通讯作者)(2013) Cloning and functionalcharacterization of the swine (Sus scrofa)organic anion transporting polypeptide 1a2. Xenobiotica. 43(8):738-44. (IF: 2.1015yr IF: 2.212, JCR: 四区)
15.  YaoJHong, W, Huang, J,Zhan K, Huang H, Hong, M (通讯作者)(2012) N-glycosylationdictates proper processing of organic anion transporting polypeptide 1B1. PLoS One. 7(12):e52563. (IF3.73, 5yr IF: 3.535, JCR: 三区)
16.  Li N, Hong W, Huang H, Lu H, Lin G, HongM (通讯作者)(2012) Identification of aminoacids essential for estrone-3-sulfate transport within transmembrane domain 2of organic anion transporting polypeptide 1B1.PLoS One. 7(5):e36647.(IF3.73, 5yr IF: 3.535, JCR: 三区)
17.  Wen G,Hong M (co-first author), Li B,Liao W,Cheng SK,Hu B,Calaf GM,Lu P,Partridge MA,Tong J,Hei TK  (2011) Transforming growthfactor-β-induced protein (TGFBI) suppresses mesothelioma progression throughthe Akt/mTOR pathway. Int J Oncol. 39(4):1001-9. IF2.57,5yr IF: 2.99, JCR: 三区)
18.  Wen G,Partridge MA,Li B,Hong M, Liao W,Cheng SK,Zhao Y,Calaf GM,Liu T,Zhou J,Zhang Z,Hei TK.(2011) TGFBI expressionreduces in vitro and in vivo metastatic potential of lung and breast tumorcells. Cancer Lett. 308(1):23-32. IF4.86, 5yr IF: 5.174,JCR: 二区)
19.  Hong,M., Xu, A., Zhou, H., Wu, L.,Randers-Pehrson, G, Santella, R, Yu, Z., and Hei, T. K. (2010)Mechanism of genotoxicity induced by targeted cytoplasmic irradiation. Brit. J.Cancer. 103(8):1263-8.IF5.045yrIF: 5.617, JCR: 二区)
20. Hong,M., Li, S.(co-first author), Zhou,F., Xu, W., Du, W., Thomas, P.E., You, G. (2010) Putative transmembrane domain12 of the human organic anion transporter hOAT1 determines transporter stability and maturation efficiency. J. Pharm. Expt.Thera. 332(2):650-8. IF3.82,5yr IF: 3.718, JCR: 二区)
21. Wen, G., Hong, M. (co-first author), Patridge, M.A., Li, B., Calaf, G.M., Hei, T.K.(2010)Phosphoproteomic profiling of arsenite-exposed human small airway epithelial cells.Oncol Rep.23(2):405-12. (IF: 2.297, 5y IF: 2.488, JCR: 四区)
22. Zhou,H., Hong, M., Chai, Y., Hei, T.K. (2009) Consequences of cytoplasmicirradiation: Studies from microbeam. J Radiat Res (Tokyo). Suppl A:A59-65.
23. Zhang, Q, Hong, M,Duan, P, Pan, Z, Ma, J, You, G (2008) Organic anion transporter OAT1 undergoes constitutiveand protein kinase C-regulated trafficking through a dynamin- and clathrin-dependentpathway J. Biol Chem. 283(47):32570-9.
24. Wen, G.,Partridge, M.A., Calaf, G.M., Meador, J.A., Hu, B., Echiburu-Chau ,C., Hong,M. and Hei T.K. (2009) Increased susceptibility of human small airwayepithelial cells to apoptosis after long term arsenate treatment. Sci Total Environ. 407(3):1174-81. Epub2008 Oct 31.
25. HeiTK, Zhou H, Ivanov VN, Hong M, Lieberman HB, Brenner DJ, Amundson SA,Geard CR (2008) Mechanism of radiation-induced bystander effects: a unifyingmodel. J Pharm Pharmacol.60(8):943-50.
26. Hong, M., Zhou, F., Lee, K., You, G. (2007) The putativetransmembrane segment 7 of human organic anion transporter hOAT1 dictatestransporter substrate binding and stability. J.Pharmacol. Exp. Ther. 320:1209-1215.
27. Hong M, Tanaka K, Pan Z, Ma J, You G. (2007) Determinationof the external loops and the cellular orientation of the N- and the C-terminiof the human organic anion transporter hOAT1. Biochem. J. 410:515-520.
28. Zhou,F., Hong, M., You, G. (2007) Regulationof human organic anion transporter 4 (hOAT4) by progesterone and protein kinaseC in human placental BeWo cells. Am J Physiol Endocrinol Metab. 293(1):E57-61.
29. Hong,M.,Xu, W., Yoshida, T., Tanaka, K., Wolff, D.J., Zhou, F, Inouye, M, and You, G.(2005) Human organic anion transporter hOAT1 forms homooligomers. J.Biol. Chem. 280:32285-32290.
30. Zhou,F., Xu, W., Hong, M., Pan, Z., Sinko, P.J., Ma, J., You, G. (2005) Therole of N-linked glycosylation in protein folding, membrane targeting, andsubstrate binding of human organic anion transporter hOAT4. Mol Pharmacol. 67:868-76.
31. Hong,M.,Zhou, F. and You, G. (2004) Critical amino acid residues in transmembranedomain 1 of the human organic anion transporter hOAT1. J. Biol. Chem. 279:31478-31482.
32. Hong,M.,Zilinskas, B.A., Knipple, D.C. and Chin, C.K. (2004) Cis-3-hexenalproduction in tobacco is stimulated by 16-carbon monounsaturated fatty acids. Phytochem. 65:159-168.
33. 黄九九,李楠,余业进,洪梅 (通讯作者)(2010)有机阴离子转运多肽及其对药物吸收、分布和排出的影响。 今日药学。20(12):1-4
34. 黄九九,洪梅(通讯作者)(2009)肿瘤多药耐药性中的ABC转运蛋白及其调节。 现代生物医学进展。9(23):4571-73.
35. 战凯,姚涓,肖云鹏, 于宣,洪梅(通讯作者)(2014) 肿瘤放化综合治疗中的靶向研究。现代生物医学进展。14(2):386-388.