张玲华:教授 博士生导师 广东省千百十培养对象  性别: 女

所属单位:生物化学及分子生物学系 
Email:lhzhang@scau.edu.cn  


个人简介
      2006年至今已在Vaccine Molecular ImmunologyDevelopmental and Comparative Immunology等国际知名期刊发表30余篇论文,累积影响因子118.9988项发明专利授权。作为学科带头人,积极推动学院“211”学科建设,协助组织并顺利通过了生化与分子生物学广东省重点学科中期评估。此外还兼任广东省科技咨询专家、教育部博士后基金评审专家等。
研究方向
微生物及免疫学
代表性成果(近五年的论文、专利等,2007年起)

1Cai, H.M., Wei, J.T., Shen, H.K., Li, J.Y., Fan, Q., Zhao, Z.Z., Deng, J.B., Ming, F.P., Zeng, M., Ma, M.P., Zhao, P.J., Liang, Q.Y., Jia, J.H., Zhang, S.X., Zhang L.H.*(张玲华). (2020). Molecular cloning, characterization and expression profiles of Annexin family (ANXA1~A6) in yellow catfish (Pelteobagrus fulvidraco) and ANX regulation by CpG ODN responding to bacterial infection. Fish & Shellfish Immunology 99, 609-630.(*通讯作者,2019 SCI Impact factor, 3.298)

2Deng J.B., Li J.Q., Ma M.P., Zhao P.J., Ming F.P., Lu Z.P., Shi J.Q., Fan Q, Liang Q.Y., Jia J.H., Li J.Y., Zhang S.X., Zhang L.H.*(张玲华).2020Co-expressing GroEL-GroES, Ssa1-Sis1 and Bip-PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone. Microb Cell Fact. 19,35.(*通讯作者,2019 SCI Impact factor, 4.402)

3Zhang S.X., Wu L., Chen J.W., Wei J.T., Cai H.M., Ma M.P., Zhao P.J., Ming F.P., Jia J.H., Li J.Y., Fan Q., Liang Q.Y., Deng J.B., Zeng M., Zhang L.H.*(张玲华).(2019) Effects of porcine IL-17B and IL-17E against intestinal pathogenic microorganism. Molecular Immunology.116:151-159(*通讯作者,2018 SCI Impact factor,3.064)

4Zhang S.X, Cai H.M., Cao D, Deng J.B., Jia J.H., Li J.Q., Ming F.P., Zhao P.J., Ma M.P., Liang Q.L., Zeng M., Zhang L.H.(张玲华)*. (2019) Recombinant plasmids containing CpG with porcine host defense peptides (PR-39/pBD-1) modulates the innate and adaptive intestinal immune responses (including maternal-derived) in piglets. Int. Immunopharmacol. 70, 467-476 (*通讯作者,2018 SCI Impact factor, 3.361)

5Cai H.M., Deng J.B., Li J.Q., Ma M.P., Huang C, Zhao P, Ming F.P., Liang Q.Y., Jia J.H., Zhang S.X., Zeng M., Zhang, L.H. (张玲华)* (2019) Modulating the 3 end-DNA and the fermentation process for enhanced production and biological activity of porcine interferon-gamma. PLoS ONE 14(3), e0214319. (*通讯作者,2018SCI Impact factor, 2.766)

6Yang, J., Cai, H.M., Liu, J., Z, Zeng. M., Cheng, Q.M, Zhang, L.H. (张玲华)*. (2018) Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts. Scientific Reports, 8:1401-1412.*通讯作者,2018 SCI Impact factor, 4.602

7Cai, H.M., Chen, J.W., Liu, J., Ming, F.P., Lu, Z.P., Cheng, Q.M., Yang, J., Zhang, L.H. (张玲华)*. (2017) CRIP1, a novel immune-related protein, activated by Enterococcus faecalis in porcine gastrointestinal epithelial cells. Gene, 598, 84-96. (*通讯作者,2016 SCI Impact factor, 2.339)

8Yang, J., Lu, Z.P., Chen, J.W., Chu, P.P., Cheng, Q.M., Liu, J., Ming, F.P., Huang, C.Y., Xiao, A.J., Cai, H.M., Zhang, L.H. (张玲华)*. (2016) Effect of cooperation of chaperones and gene dosage on the expression of porcine PGLYRP-1 in Pichia pastoris. Applied Microbiology and Biotechnology, 100:54535465. (* 通讯作者, 2015 SCI Impact factor, 3.811)

9Cai, H.M., Kuang Z.S., Huang K.Y., Shi, J.Q., Zhao X.J., Chu, P.P., Huang, C.Y., Ming, F.P., Xia, F.G., Yang, J., Zhang, L.H. (张玲华)*. (2014) CpG oligodeoxynucleotide protect neonatal piglets from challenge with the enterotoxigenic E. coli , Veterinary Immunology and Immunopathology, 161, 6676 (* 通讯作者2013 SCI Impact factor, 1.782)

10Ming, F.P., Yang, J., Chu, P.P., Ma, M.P., Shi, J.Q., Cai, H.M., 参与人, Li, H.Z., Jiang, Z.G., Wang, W.F., Zhang, S.Q., Zhang L.H.(张玲华)*. (2013) Immunization of Aged Pigs with Attenuated Pseudorabies Virus Vaccine Combined with CpG Oligodeoxynucleotide Restores Defective Th1 Immune Responses, PLOS ONE,  8(6), 65536-65536 (* 通讯作者,2013 SCI Impact factor, 4.092)

11Ma, M.P., Wang, L.L., Yang, J., Cai, H.M., Shi, J.Q., Huang, Z.F., Zhang, S.Q., Zhang L.H.(张玲华)*. (2012) Age-related impaired Th1 responses to PRV vaccine in vivo in aged pigs, Molecular Immunology, 52, 217-222 (* 通讯作者,2011 SCI Impact factor, 2.897)

12Yang, J., Mao, M.P., Huang Z.F., Zhang, S.Q., Chen, S.F., Li, H.Z., Jiang, Z.G., Cao, G.J., Cao, D., Wang, X.F., Zhang L.H.(张玲华)*. (2012)  Innate defence regulator peptide synergizes with CpG ODN for enhanced innate instestinal immune responses in neonate piglets. Int. Immunopharmacol. 12(2), 415424 (*通讯作者,2011 SCI Impact factor, 2.325)

13Cao, D., Li, H.Z., Jiang, Z.G, Cheng, Q., Yang, Z.H., Xu, C.C., Cao, G.J., Zhang L.H.(张玲华)*. (2011) CpG oligodeoxynucleotide synergizes innate defense regulator peptide for enhancing the systemic and mucosal immune responses to pseudorabies attenuated virus vaccine in piglets in vivo.  Int. Immunopharmacol. 11, 748754 (*通讯作者,2010 SCI Impact factor, 2.325)

14  Cao, D., Li, H.Z., Jiang, Z.G, Xu, C.C., Cheng, Q., Yang, Z.H., Cao, G.J., Zhang L.H.(张玲华)*. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets. Vaccine. 28, 60066013. (*通讯作者,2009 SCI Impact factor, 3.616)

15Cheng, Q., Xu, C.C., Zhang L.H.(张玲华)*, Li, J.Q., Cao, T., Zhang, M.R. (2010) Administered CpG Oligodeoxynucleotide Induces mRNA expression of CXC and CC Chemokines at the Intestinal Mucosa and PBMCs in Piglets. Int. Immunopharmacol. 10, 611-618 (*通讯作者, 2009 SCI Impact factor, 2.214)

16Cheng, Q., Jiang, Z.G.,  Xu, C.C., Li, H.Z., Cao, D., Yang, Z.H., Cao, G.J., Zhang L.H.(张玲华)* (2010)  CpG oligodeoxynucleotide promotes protective immunity in the enteric mucosa and suppresses enterotoxigenic E. coli in the weaning piglets. Int. Immunopharmacol.   10, 12491260 (*通讯作者, 2009 SCI Impact factor, 2.214)

17Zhang L.H.(张玲华)*, Tian, X.S., Zhou, F.Z. (2008a) In vivo oral administration effects of variousoligodeoxynucleotides containing synthetic immunostimulatory motifs in the immune response to pseudorabies attenuated virus vaccine in newborn piglets. Vaccine. 26, 224-233. (*通讯作者,2007 SCI Impact factor, 3.377)

18Zhang L.H.(张玲华)*, Zhang, M.R., Li, J.Q., Cao, T., Tian, X.S., Zhou, F.Z. (2008b) Enhancement of mucosal immune responses by intranasal co-delivery of Newcastle disease vaccine plus CpG oligonucleotide in SPF chickens in vivo.  Research in Veterinary Science. 85,  495–502.  (2007 SCI Impact factor, 1.274)

19Zhang L.H.(张玲华)*, Tian, X.S., Zhou, F.Z. (2007a)  In vivo immunostimulatory effects of CpG ODN in newborn piglets. Molecular Immunology. 44, 12381244. (*通讯作者,2006 SCI Impact factor, 4.768)

20Zhang L.H.(张玲华)*, Tian, X.S., Zhou, F.Z. (2007b) In vivo effects of oligodeoxynucleotides containing synthetic immunostimulatory motifs in the immune response to swine streptococcic septicemia vaccine in weaned piglets. Molecular Immunology. 44, 11411149. (*通讯作者,2006 SCI Impact factor, 4.768)

21Zhang L.H.(张玲华)*, Tian, X.S., Zhou, F.Z. (2007c) Vaccination with porcine reproductive and respiratory syndrome killed virus vaccine and immunostimulatory oligodeoxynucleotides induces specific immunity in piglets. Vaccine. 25, 17351742. (*通讯作者,2006 SCI Impact factor, 3.159)

22Zhang L.H.(张玲华)*, Tian, X.S., Zhou, F.Z. (2007d) Vaccination with Newcastle disease vaccine and CpG oligodeoxynucleotides induces specific immunity and protection against Newcastle disease virus in SPF chicken. Veterinary Immunology and Immunopathology. 115, 216222. (*通讯作者,2006 SCI Impact factor, 1.994)

23Zhang L.H.(张玲华)*, Tian, X.S., Zhou, F.Z. (2007e) Intranasal administration of CpG oligonucleotides induces mucosal and systemic Type 1 immune responses and adjuvant activity to porcine reproductive and respiratory syndrome killed virus vaccine in piglets in vivo. International Immunopharmacology. 7(13), 17321740. *通讯作者, 2006 SCI Impact factor, 2.157

24Zhang L.H.(张玲华)*, Tian, X.S., Zhou, F.Z. (2007f) CpG oligodeoxynucleotides augment the immune responses of piglets to swine Pasteurella multocida living vaccine in vivo. Research in Veterinary Science. 83(2),171-181. (2006 SCI Impact factor, 1.258)

25Zhang L.H.(张玲华)*, Guo, Y., Tian, X.S., Zhou, F.Z. (2006a) Co-administration of porcine-specific CpG oligodeoxynucleotide enhances the immune responses to pseudorabies attenuated virus vaccine in newborn piglets in vivo. Developmental and Comparative Immunology. 30(6), 589-596. (*通讯作者,2005 SCI Impact factor, 3.261)

26Zhang L.H.(张玲华)*, Tian, X.S., Guo, Y., Zhou, F.Z. (2006b) Effects of CpG ODN on CD4+ and CD8+ T subpopulations in the immune response to porcine reproductive and respiratory syndrome killed virus vaccine. Vaccine. 24(11), 1874-1879 . (*通讯作者,2005 SCI Impact factor, 2.822)

27Zhang L.H.(张玲华)*, Tian, X.S., Zhou, F.Z. (2006c) The efficacy of CpG oligodinucleotides, in combination with conventional adjuvants, as immunological adjuvants to swine streptococcic septicemia vaccine in piglets in vivo. International Immunopharmacology. 6(8), 1267-1276. (*通讯作者,2005 SCI Impact factor, 2.008)

28Zhang L.H.(张玲华)*, Tian, X.S., Zhou, F.Z. (2006d) In vivo effects of oligodeoxynucleotides containing synthetic immunostimulatory motifs in weaned piglets. International Immunopharmacology. 6(10),1623-1631. (*通讯作者,2005 SCI Impact factor, 2.008)

29Zhang L.H.(张玲华)*, Guo, Y., Tian, X.S., Zhou, F.Z. (2006e) CpG oligodinucleotides induce strong humoral and cellular responses to swine streptococcic septicemia vaccine in piglets in vivo. International Immunopharmacology. 6(3), 342-350. (*通讯作者,2005 SCI Impact factor, 2.008)

30Zhang L.H.(张玲华)*, Tian, X.S., Guo, Y., Zhou, F.Z. (2006f) The effect of transgenic expression of porcine interleukin-6 gene and CpG sequences on immune responses of the newborn piglets inoculated with pseudorabies attenuated vaccine. Research in Veterinary Science. 80, 281-286. (2005 SCI Impact factor, 1.106)

相关专利

(1)张玲华,田兴山,邝哲师,陈薇,李健雄,有益微生物制剂稳定化预处理工艺,2006.12,中国,ZL200310117512.8
(2)邝哲师,田兴山,张玲华,陈薇,李健雄,“有益微生物制剂稳定化后处理工艺”, 2006.8,中国, ZL200310117511.3
(3)邝哲师,张玲华,徐志宏,“一种微生物转化菠萝渣为生物饲料的制备方法”, 2008.1,中国, ZL200610033024.2
(4)张玲华,李华周,姜增固。“一种高效CpG制剂及其制备方法和应用”, 2014.6,中国,ZL201210090979.7
(5)张玲华,邝哲师,蔡海明,赵祥杰,楚品品,叶明强,“一种高效提高枯草芽孢杆菌出芽率的方法”,专利号:ZL201410162370.5
(6)张玲华,蔡海明,马苗鹏,周洁安,“一种高效抗炎性PB ODN序列及其应用”,专利号:ZL 20151043403.5
(7)张玲华,马苗鹏,杨军,“一种嗜酸乳酸杆菌与醋酸杆菌混合发酵的方法”,专利号:ZL201510335715.7
(8)江学斌,张玲华,胡位荣,马苗鹏,黄瀚威,“一种高效降低水体COD沼泽红假单胞菌的培养基”专利号:ZL201610026084.5

承担课题

(1) 横向项目,新型裂裥菌素-Toll5激动剂纳米生物材料研制及应用示范、2019/10-2022/10、200万元、在研、主持。
(2) 广州市重点科技项目,新型Plectasin分泌型益生菌剂高效防治罗非鱼链球菌病研发及应用,2019/01-2022/12、45万元、在研、主持。
(3) 广东省自然科学基金自由申请项目,CRIP1通过调节Zn2+水平控制猪肠道沙门氏菌感染的机制研究、 2018/01-2021/12、10万元、在研、主持。
(4)广州市重点科技项目,3-OH PAME淬灭酶分泌型高效青枯病复合生防菌剂研发及应用, 2018/01-2021/12、45万元、在研、主持。
(5)广东省自然科学基金自由申请项目,HPS感染细胞模型的建立及新型Sup ODN抗HPS感染机制研究、 2015/01-2017/12、10万元、在研、主持。
(6)广州市产学研协同创新重大专项目,益生菌-荔枝多酚对仔猪腹泻的防治研究,2016/01-2019/12、100万元、在研、合作主持。
(7)2012.1-2014.12 主持广东省教育部产学研结合项目“分泌型毕赤酵母发酵制备饲用抗菌肽 PR39 的研究及应用”( 30万元,NO:2011B090300020,已完成)
(8)2011.1-2013.12 主持国家自然科学基金“CpG ODN 通过NF-κB 信号系统延缓T 细胞衰老的分子机制”(34万元,NO:31072114,已完成)
(9)2010.1-2012.12 主持广州市重点科技项目“抗仔猪断奶应激腹泻CpG制剂关键技术研究及示范”(15万元,NO: 2010Z1-E311,已完成)
(10)2009.9-2012.9  主持广东省农业攻关项目“幼猪高热病疫苗免疫增效剂CpG的研究及应用 ”(5万元,NO:  2009B020307010 ,已完成)
(11)2008.8-2010.12 主持美国Merial合作项目“CpG仔猪抗病免疫机理研究”(24万元,已完成)
(12)2008.1-2010.12 主持广州市重点科技项目“高效功能型硝酸盐降解菌群的构建及其制剂的生产和应用”,(12万元,NO:2008-Z1-E081,已完成)
(13)2007.10-2008.9 主持美国Merial合作项目“CpG诱导仔猪先天性免疫能力”(51.3万元,已完成)
(14)2006.9-2009.8 主持广东省农业攻关项目“高效硝酸盐降解功能菌群的构建及应用”(6万元,NO: 2006B20601011,已完成)

获奖成果

(1)田兴山,张玲华,石和荣,邝哲师,李健雄,柯浩,潘木水,唐小俊,马江耀,陈薇,黄晓光,谢海燕,周风珍,李国立,孙晓刚. “有益微生物制剂促进动物生长的研究及应用” ,2005获广东省科技进步二等奖。
(2)田兴山, 石和荣,张玲华,张彪,邝哲师,唐小俊,柯  浩,李健雄,马江耀,周风珍,陈薇. “对虾生态养殖技术研究及应用”, 2005年度获得茂名市科技进步三等奖。