洪梅

发布者:生命科学学院发布时间:2018-08-09浏览次数:8271


        教授  性别: 女

        所属单位:生物化学系

 

        Email:mh2788@scau.edu.cn

  


研究兴趣

  • 药物转运蛋白的结构-功能关系

  • 药物转运蛋白在兽药吸收、分布和排除(ADME)中的作用及其调控 

  • 膜蛋白在对外界环境改变的反应中的信号传导作用


学历

  • 1998.9-2003.12     罗格斯大学植物学与植物病理学系 (Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Jersey, USA), 博士

  • 1992.9-1996.7       中山大学化学系,学士


工作经历

2009.7月至今          华南农业大学生命科学学院 教授

2007.7-2009.6        哥伦比亚大学医学院辐射研究中心 (Center for Radiological Research, Columbia University Medical Center, New York, NY, USA)  博士后研究科学家

2006.12-2007.6      劳伦斯伯克莱国家实验室生物学分部 (Life science division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA)  访问博士后学者

2003.12-2006.12    罗格斯大学药学院药学系 (Department of Pharmaceutics, College of Pharmacy, Rutgers, The State University of New Jersey, New Jersey, USA) 博士后/助理研究员/副研究员


讲授课程

本科生授课:

  • 动物生物化学 (双语授课)

  • 生物化学A(双语授课)

  • 生物化学C

  • 生物化学D(双语授课)

  • 生物化学与分子生物学(双语授课)

  • 生化与分子生物学专题

  • 生物技术进展

  • 生物专业英语

研究生授课: 

  • 生物化学与分子生物学专业英语

  • 生物化学与分子生物学文献综述

  • 生化与分子生物学进展

  • 高级生物化学


实验室研究项目

      1.人体转运蛋白的结构-功能关系研究

      药物转运蛋白和药物代谢酶,药物目标的共同作用决定了人体对许多药物的反应。因此,药物转运是生物体对药物吸收的关键环节。转运蛋白功能的缺失,会造成人体药物排除或解毒障碍,从而造成药物诱导的肝或肾衰竭。近年来,由于认识到转运蛋白在药物转运中的重要作用,许多大型跨国药厂已把对这类蛋白在药物动力学和药效学上的研究作为药物研发中的重要一环。然而,迄今为止,对它们的结构-功能关系的研究只有不多的报道。本实验室利用各种生化技术,开展对药物转运蛋白结构-功能关系的系统研究,鉴定其序列中起关键作用的氨基酸,探索它们翻译后调节控制机理。

      2.药物转运蛋白在兽药吸收和排除中的作用

      随着畜牧业的现代化、集约化和规模化生产,兽药及兽药添加剂在降低发病率与死亡率、提高饲料利用率、促进生长和改善肉品品质方面起着十分显著的作用,已成为现代畜牧业发展不可缺少的物质基础。但是,由于饲养技术的落后和经济利益的驱使,养殖过程中滥用兽药和超标使用兽药的现象普遍存在。各种药物及激素残留对于人体健康和生态环境所带来的不良影响日益引起关注。对药物的吸收效率,是由吸收转运蛋白和外排转运蛋白协同作用而共同决定的。通过系统研究转运蛋白在食品动物药物吸收和排泄中的作用,将能填补吸收转运蛋白在兽药生物利用度中所起的作用方面的空白,为有效地提高兽药的吸收分布提供重要的理论依据。

      3. 膜蛋白在信号传导中的作用

      膜蛋白是否能正确地表达在特定的膜结构中是其发挥正常功能的关键因素,膜蛋白的翻译后修饰,在细胞器如内质网、高尔基体中的处理过程受多种因素的影响。细胞通过调控膜蛋白在细胞膜表面的表达量和表达位点,对各种外界环境变化(如营养元素量的改变、外界刺激等)作出反应。实验室的另一研究方向是以生物化学和细胞生物学方法,研究膜蛋白在信号传导过程的重要作用,探索其所介导的细胞对外界刺激如辐射、环境毒物的反应。 

发表论文

专著章节:

Hong, M., You, G. (2007) Regulation of drug transporter activity (chapter 17, pp517-533) in Drug Transporters: Molecular Characterization and Role in Drug Disposition. Eds Guofeng You, Marilyn Morris and Binghe Wang. John Wiley & Son Inc 


论文:

1.Chen J, Xue Y, Shuai X, Ni C, Fang Z, Ye L, Hong M* (2022) Effect of major components of Tripterygium wilfordii Hook. f on the uptake function of organic anion transporting polypeptide 1B1. Toxicology and Applied Pharmacology. 435:115848

2.Ni C, Wang X, Chen J, Xu S, Ye W, Hong M* (2021) Leucine heptad motifs within transmembrane domains affect function and oligomerization of human organic anion transporting polypeptide 1B1. Biochim Biophys Acta Biomembr 2021 Apr 1;1863(4):183554. doi: 10.1016/j.bbamem.2021.183554. Epub 2021 Jan 8.

3.Fang Z, Wang Y, Wang Z, Xu M, Ren S, Yang D, Hong M*, Xie W* (2020) ERINA is an estrogen-responsive lncRNA that drives breast cancer through the E2F1/RB1 pathway. Cancer Res. 2020 Aug 21; canres.1031.2020. doi: 10.1158/0008-5472.CAN-20-1031. 2020 Oct 15;80(20):4399-4413.

4.Wang Y, Fang Z, Hong M, Yang D, Xie W* (2020) Long-noncoding RNAs (lncRNAs) in drug metabolism and disposition, implications in cancer chemo-resistance. Acta Pharmaceutica Sinica B 10(1):105-112. Available online 19 October 2019

5.Xu S, Chen G, Liu Z, Xu D, Wu Z, Li Z, Hong M* (2019) Site-directed mutagenesis reveals crucial residues in Escherichia coli Resistance-Nodulation-Division efflux pump OqxB. Microb Drug Resist. 2019 Dec 13. doi: 10.1089/mdr.2019.0165. 2020 Jun;26(6):550-560. [Epub ahead of print]

6.He S, Zhang W, Zhang X, Xu P, Hong M, Qu S*. (2019) The 4b-4c loop of excitatory amino acid transporter 1 containing four critical residues essential for substrate transport. J Biomol Struct Dyn. 2019 Sep 15:1-11. doi: 10.1080/ 07391102.2019.1664935. [Epub ahead of print]

7.Lin X, Xiang Z, Wang B, Chen H, Zhou T, Hong M* (2019) Interaction of swine organic anion transporting polypeptide 1a2 with tetracycline, macrolide and β-lactam antibiotics. Toxicol Appl Pharmacol. 2019 Jul 6; 379:114649. doi: 10.1016/j.taap.2019.114649. [Epub ahead of print]

8.Wang X, Chen J, Xu S, Ni C, Fang Z, Hong M* (2019) Amino-terminal region of human organic anion transporting polypeptide 1B1 dictates transporter stability and substrate interaction. Toxicol Appl Pharmacol. 2019 Jun 27;378:114642. doi: 10.1016/j.taap.2019.114642. [Epub ahead of print]

9.Wang X, Liang Y, Fang Z, Huang J, Hong M* (2019) The intracellular NPxY motif is critical in maintaining the function and expression of human organic anion transporting polypeptide 1B1. Biochim Biophys Acta Biomembr. 2019 Jun 1;1861(6):1189-1196. doi: 10.1016/j.bbamem.2019.04.001. Epub 2019 Apr 7.

10.Fang Z, Huang J, Chen J, Xu S, Xiang Z, Hong M* (2018) Transmembrane domain 1 of human organic anion transporting polypeptide 2B1 is essential for transporter function and stability. Mol Pharmacol. 2018 Aug;94(2):842-849. doi: 10.1124/mol.118.111914. Epub 2018 Jun 5.

11.Xiang Z, Li W, Wang L, Yi J, Chen K, Hong M* (2018) Identification of a NFκB inhibition site on the proximal promoter region of human organic anion transporting polypeptide 1A2 coding gene SLCO1A2.Drug Metab Dispos. 46(5):643-651. Epub 2018 Mar 16.

12.Zhou C, Rong Y, Konishi T, Xiang Z, Zihui F, Hong M*2017Effect of carbon-ion radiation on drug transporters organic anion transporting polypeptides in breast cancer cells. Radiat Res. 187(6):689-700. 2017 Apr 7.

13.Ni C, Yu X, Fang Z, Huang J, Hong M* (2017) Oligomerization study of human organic anion transporting polypeptide 1B1.Mol Pharmceutic. 14(2):359- 367. Epub 2017 Jan 12

14.Hong M (2017) Biochemical studies on the structure-function relationship of major drug transporters in the ATP-binding cassette family and solute carrier family. Adv Drug Deliv Rev. 116(2017): 3-20. 2017 Jul 1; Epub 2016 Jun 15. pii: S0169-409X(16)30187-9. doi: 10.1016/j.addr.2016.06.003.

15.Fang Z, Xu A, Wu L, Hei TK, Hong M* (2016) The role of protein kinase C alpha translocation in radiation-induced bystander effect. Sci Rep. 2016 May 11;6:25817.

16.Hong W, Wu Z, Fang Z, Huang J, Huang H, Hong M* (2015) Amino acid residues in the putative transmembrane domain 11 of human organic anion transporting polypeptide 1B1 dictate transporter substrate binding, stability, and trafficking. Mol Pharmaceut. 2015 Dec 7;12(12):4270-6. 2015 Nov 20.

17.Hong M*, Hong W, Ni C, Huang J, Zhou C.(2015) Protein kinase C affects the internalization and recycling of organic anion transporting polypeptide 1B1. Biochim Biophys Acta. 1848(10 Pt A):2022-2030.2015 May 22.

18.Hu W, Xu S, Yao B, Hong M, Wu X, Pei H, Chang L, Ding N, Gao X, Ye C, Wang J, Hei TK, Zhou G.2014MiR-663 inhibits radiation-induced bystander effects by targeting TGFB1 in a feedback mode. RNA Biol. 11(9):1189-98

19.Xiao Y, Deng J, Liu X, Huang J, Sun Y, Dai R, Hong M * (2014) Different binding sites of bovine organic anion-transporting polypeptide1a2 are involved in the transport of different fluoroquinolones. Drug Metab Dispos. 42(8):1261-7.

20.Hong M. (2014) Heavy ion-related bystander effects. Rend. Fis. Acc. Lincei 25(1 supp): 65-68.

21.Hong M. (2014) Critical domains within the sequence of human organic anion transporting polypeptides. Curr Drug Metab. 15(3):265-70. 2013 Dec 28.

22.Huang J, Li N, Hong W, Zhan K, Yu X, Huang H, Hong M.* (2013) Conserved tryptophan residues within putative transmembrane domain 6 affect transport function of organic anion transporting polypeptide 1B1.Mol Pharmacol. 84(4):521-7.

23.Zhan K, Yao J, Huang J, Zhou G, Li W, Chen L, Hong M* (2013) Effect of X-ray irradiation on expression of organic anion transporting polypeptides. Int J Radiat Biol. 89(10):832-40 .

24.Liu, X, Huang, J, Sun, Y, Zhan, K, Zhang, Z, Hong, M* (2013) Identification of multiple binding sites for substrate transport in bovine organic anion transporting polypeptide 1a2. Drug Metab Dispos, 41(3):602-607, Epub 2012 Dec 19.

25.Yu, Y, Liu, X, Zhang, Z, Xiao, Y, Hong, M* (2013) Cloning and functional characterization of the swine (Sus scrofa) organic anion transporting polypeptide 1a2. Xenobiotica. 43(8):738-44.

26.Yao JHong, W, Huang, J, Zhan K, Huang H, Hong, M* (2012) N-glycosylation dictates proper processing of organic anion transporting polypeptide 1B1. PLoS One. 7(12): e52563.

27.Li N, Hong W, Huang H, Lu H, Lin G, Hong M* (2012) Identification of amino acids essential for estrone-3-sulfate transport within transmembrane domain 2 of organic anion transporting polypeptide 1B1.PLoS One. 7(5):e36647.

28.Wen G, Hong M (co-first author), Li B, Liao W, Cheng SK, Hu B, Calaf GM, Lu P, Partridge MA, Tong J, Hei TK  (2011) Transforming growth factor-β-induced protein (TGFBI) suppresses mesothelioma progression through the Akt/mTOR pathway. Int J Oncol. 39(4):1001-9.

29.Wen G, Partridge MA, Li B, Hong M, Liao W, Cheng SK, Zhao Y, Calaf GM, Liu T, Zhou J, Zhang Z, Hei TK.(2011) TGFBI expression reduces in vitro and in vivo metastatic potential of lung and breast tumor cells. Cancer Lett. 308(1):23-32.

30.Hong,M., Xu, A., Zhou, H., Wu, L., Randers-Pehrson, G, Santella, R, Yu, Z., and Hei, T. K. (2010) Mechanism of genotoxicity induced by targeted cytoplasmic irradiation. Brit. J. Cancer. 103(8):1263-8.

31.Hong, M., Li, S.(co-first author), Zhou, F., Xu, W., Du, W., Thomas, P.E., You, G. (2010) Putative transmembrane domain 12 of the human organic anion transporter hOAT1 determines transporter stability and maturation efficiency. J. Pharm. Expt. Thera. 332(2):650-8.

32.Wen, G., Hong, M. (co-first author), Patridge, M.A., Li, B., Calaf, G.M., Hei, T.K.(2010) Phosphoproteomic profiling of arsenite-exposed human small airway epithelial cells. Oncol Rep.23(2):405-12.