吴骏

发布者:生命科学学院发布时间:2018-08-09浏览次数:4098


      副教授  性别:男

      所属单位:生物化学系

      Email:wujun@scau.edu.cn

  



工作经历

2018/01-现在,华南农业大学,生命科学学院生化与分子生物学系,副教授
2006/08-2017/12,华南农业大学,生命科学学院生化与分子生物学系,讲师


主要研究领域

研究方向是外源化合物(药物、毒素)的代谢转化机制,主要利用计算生物学(分子对接和分子动力学模拟),生物化学与分子生物学及酶学的方法研究外源化合物(药物、毒素)被人或动物体内代谢关键酶催化的分子机制。


主持的科研课题

1. 广州市科技计划项目,201607010178,黄曲霉毒素B1在猪肝脏代谢关键酶的底物选择和调控的分子机制,2016/04-2019/03,20万,已结题,主持
2. 国家自然科学基金青年基金,31101852,黄曲霉毒素在猪体内代谢的关键细胞色素P450氧化酶的筛选、确定及催化机制研究,2012/01-2014/12,23万元,已结题,主持
3. 科技部“973”计划课题,2009CB118802,受试化合物在畜禽代谢关键酶的功能及结构研究, 2009/01-2013/12,412万元,已结题,参加


发表的主要研究论文

1. Jun Wu#, Sisi Zhu#, Yunbo Wu, Tianqing Jiang, Lingling Wang, Jun Jiang, Jikai Wen, and Yiqun Deng. Multiple CH/π interactions maintain the binding of aflatoxin B1 in active cavity of human cytochrome P450 1A2. Toxins, 2019, 11(3): 158.
2. Sisi Zhu
#, Jun Wu#, Silin Liu, Tianqing Jiang, Yiqun Deng*, Phe-125 and Phe-226 of pig cytochrome P450 1A2 stabilize the binding of aflatoxin B1 and 7-ethoxyresorufin through the key CH/π interactions. Biochemical Pharmacology 2019, 166: 292-299.
3. Lingling Wang#,
Jun Wu#, Zhiwen Liu, Yutao Shi, Jinqiu Liu, Xiaofan Xu, Shuxian Hao, Peiqiang Mu, Fengru Deng, and Yiqun Deng, Aflatoxin B1 Degradation and Detoxification by Escherichia coli CG1061 Isolated From Chicken Cecum. Frontiers in Pharmacology, 2019, 9:1548. doi: 10.3389/fphar.2018.01548
4. Haoran Jiang,
Jun Wu, Feiyong Zhang, Jikai Wen, Jun Jiang, and Yiqun Deng, The critical role of porcine cytochrome P450 3A46 in the bioactivation of aflatoxin B1. Biochemical Pharmacology 2018, 156:177-185.
5.
Jun Wu#, Ruohong Chen#, Caihui Zhang, Kangbai Li, Weiying Xu, Lijuan Wang, Qingmei Chen, Peiqiang Mu, Jun Jiang, JikaiWen and Yiqun Deng*. Bioactivation and regioselectivity of pig cytochrome P450 3A29 towards aflatoxin B1. Toxins, 2016, 8(9): 267-283.
6.
Jun Wu#, Weiying Xu#, Caihui Zhang, Qing Chang, Xianqing Tang, Kangbai Li, Yiqun Deng*. Trp266 determines the binding specificity of a porcine aflatoxin B1 aldehyde reductase for aflatoxin B1-dialdehyde. Biochemical Pharmacology, 2013, 86(9):1357-1365.
7. Xianqing Tang, Peiqiang Mu,
 Jun Wu, Jun Jiang, Caihui Zhang, Ming Zheng, Yiqun Deng*. Carbonyl Reduction of Mequindox by Chicken and Porcine Cytosol and Cloned CBR1. Drug Metabolism and Disposition 2012, 40(4): 788-795.
8. Peiqiang Mu, Ming Xu, Lei Zhang, Kaixin Wu,
Jun Wu, Jun Jiang, Qingmei Chen, Lijuan Wang, Xianqing Tang, Yiqun Deng*. Proteomic changes in chicken primary hepatocytes exposed to T-2 toxin are associated with oxidative stress and mitochondrial enhancement. Proteomics 2013, 13(21):3175-3188.
9. Jing Liu
#, Man Ouyang#, Jun Jiang, Peiqiang Mu, Jun Wu, Qi Yang, Caihui Zhang, Weiying Xu, Lijuan Wang, Michael S.Y. Huen*, Yiqun Deng*. Mequindox induced cellular DNA damage via generation of reactive oxygen species. Mutation Research-Genetic Toxicology and Environmental Mutagenesis 2012, 741(1-2): 70-75.
10. Shufeng Shang, Suiyi Tan, Kangbai Li,
Jun Wu, Huina Lin, Shuwen Liu*, Yiqun Deng*. Glu659Leu substitution of recombinant HIV fusion inhibitor C52L induces soluble expression in E. coli with equivalent anti-HIV potency. Protein Engineering Design & Selection 2011, 24(7): 545-551.
11. Shirley M.H. Sy
#, Jun Jiang#, Sui-sui Dong, Gabriel Tsz-Mei Lok, Jun Wu, Hua Cai, Enoch S.L. Yeung, Jun Huang, Junjie Chen, Yiqun Deng*, Michael S.Y. Huen*. Critical roles of ring finger protein RNF8 in replication stress responses. Journal of Biological Chemistry 2011, 286(25): 22355-22361.
12. Jing Liu, Xiaohu Ge, Man Ouyang, Xianqing Tang,
Jun Wu, Junping Wang, Jun Jiang, Michael S.Y. Huen*, Yiqun Deng*. Catalytic characteristics of CYP3A22-dependent mequindox detoxification. Catalysis Communications 2011, 12(7): 637-643.
13. Xiaohu Ge#, Junping Wang#, Jing Liu, Jun Jiang, Huina Lin,
Jun Wu, Man Ouyang, Xianqing Tang, Ming Zheng, Ming Liao, Yiqun Deng*. The catalytic activity of cytochrome P450 3A22 is critical for the metabolism of T-2 toxin in porcine reservoirs. Catalysis Communications 2010, 12(2): 71-75.
14.
Jun Wu, Fen Du, Peng Zhang, Izhar Ahmed Khan, Jie Chen, Yi Liang*. Thermodynamics of the interaction of aluminum ions with DNA: Implications for the biological function of aluminum. Journal of Inorganic Biochemistry, 2005, 99: 1145-1154.
15. Hong Xu, Yi Liang*, Peng Zhang, Fen Du, Bing-Rui Zhou,
Jun Wu, Jian-Hong Liu, Zhi-Gang Liu, Liang-Nian Ji Biophysical studies of a ruthenium(II) polypyridyl complex binding to DNA and RNA prove that nucleic acid structure has significant effects on binding behaviors. Journal of Biological Inorganic Chemistry 2005, 10(5): 529-538.