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Working Experience:
Sep, 2014 – Present | South China Agricultural University, Guangzhou, China Professor & Principle Investigator |
Dec, 2010 Aug, 2014 | Johns Hopkins University, Baltimore, MD, USA & The Chinese University of Hong Kong, Hong Kong Postdoctoral Research Scientist |
Education:
Aug, 2006 – Oct, 2010 | Ph.D., The Chinese University of Hong Kong, Hong Kong (Cell and Molecular Biology) |
Aug, 2003 – July, 2006 | M.Sc., Lanzhou University, China (Molecular Biology) |
Aug, 1999 – July, 2003 | B.Sc., Lanzhou University, China (Biology) |
Research Interests:
The research in Wang laboratory asks how pollen tube growth is empowered and regulated to accomplish plant fertilization. Distinct from dynamic and flagellated animal sperm cells, higher flowering plant sperm cells are immotile. Pollen grains are the male gametophytes and they give rise to the fast growing pollen tube that burrows through the style and into the ovary of the pistil to deliver two immotile sperm cells to the ovules wherein lies the eggs. Therefore, pollen tube growth is featured as highly dynamic and polarized cell growth. The lab aims to know how intracellular activities are spatiotemporally orchestrated to support the asymmetric growth of pollen tubes, how a dynamic growing pollen tube tip is sculptured and maintained, and how we might exploit these processes to address the mysteries of plant polarization and development. By using a combination of advanced bio-imaging, gene editing, genetic, biochemical and mathematical modeling approaches, our long-term goal is to uncover the molecular mechanisms that govern pollen tube growth for plant fertilization and reproduction. Wang lab welcomes diverse types of research collaborations and is interested in recruiting ambitious and highly motivated postdoctoral research associates and postgraduate students to join us to explore the mysteries of plant fertilization and reproduction.
Publications:
2024:
34. He Yan#, Zhen Lu# (co-first author), Xiaojuan Du, Zhengtao You, Mingkang Yang, Nianle Li, Xuequan Li, Zailue Ni, Hong Wu, Xiangfeng Wang, Lifeng Zhao and Hao Wang* (2024) Autophagy modulates Arabidopsis male gametophyte fertility by controlling actin organization. Nature Communications, (accepted and in press).
33. Xun Weng and Hao Wang* (2024) Quantifying Protein Dynamics in Growing Pollen Tubes using Kymograph Analysis. Methods in Molecular Biology 2841, 131-143.
32. He Yan#, Ao Qi# (co-first author), Zhen Lu# (co-first author), Zhengtao You# (co-first author), Ziheng Wang, Haiying Tang, Xinghai Li, Qiao Xu, Xun Weng, Xiaojuan Du, Lifeng Zhao and Hao Wang* (2024) Dual roles of AtNBR1 in regulating selective autophagy via liquid-liquid phase separation and recognition of non-ubiquitinated substrates in Arabidopsis. Autophagy, DOI: 10.1080/15548627.2024.2391725.
31. Xiaojuan Du#, Xun Weng# (co-first author), Binyang Lyu, Lifeng Zhao and Hao Wang* (2024) Localized calcium transients in phragmoplast regulate cytokinesis of tobacco BY-2 cells. Plant Cell Reports 43, DOI: https://doi.org/10.1007/s00299-024-03181-3.
30. Xiaojuan Du, Yingmiao Gao, Hao Zhang, Xiaoyu Xu, Ying Li, Lifeng Zhao*, Ming Luo* and Hao Wang* (2024) HDA6 modulates Arabidopsis pavement cell morphogenesis through epigenetic suppression of ROP6 GTPase expression and signaling. New Phytologist 241, 2523-2539.
2023:
29.Xun Weng, Yifan Shen, Liwen Jiang, Lifeng Zhao*, Hao Wang* (2023), Spatiotemporal organization and correlation of tip-focused exocytosis and endocytosis in regulating pollen tube tip growth. Plant Science 330, 111633.
28.Jean-Marc Neuhaus*, Peter Pimpl*, Qiong Zhao* and Hao Wang* (2023), Editorial: Regulation of plant organelle biogenesis and trafficking. Frontiers in Plant Science, DOI: 10.3389/fpls.2023.1211807.
2022:
27.Xun Weng and Hao Wang* (2022), Apical vesicles: social networking at the pollen tube tip. Reproduction and Breeding, 99, 536-555.
26.He Yan, Menglong Zhuang, Xiaoyu Xu, Shanshan Li, Mingkang Yang, Nianle Li, Xiaojuan Du, Kangwei Hu, Xiaomin Peng, Wei Huang, Hong Wu, Yu Chung Tse, Lifeng Zhao, Hao Wang* (2022), Autophagy and its mediated mitochondrial quality control maintain pollen tube growth and male fertility in Arabidopsis. Autophagy, DOI: https://doi.org/10.1080/15548627.2022.2095838.
25.Zhengjiang Xu, Mei Yang, Zhaoyang Li, Jian Xiao, Xueqing Yang, Hao Wang and Xiaofeng Wang* (2022) Tissue-specific pectin methylesterification and pectin methylesterase activities play a role in lettuce seed germination. Scientia Horticulturae 301,111134.
2020:
24.Lifeng Zhao, Muhammad Saad Rehmani and Hao Wang* (2020) Exocytosis and endocytosis: yin-yang crosstalk for sculpting a dynamic growing pollen tube tip. Frontiers in Plant Science, DOI: 10.3389/fpls.2020.572848.
2019:
23.Shanshan Li, He Yan, Wei-Ming Mei, Yu Chung Tse* and Hao Wang* (2019) Boosting autophagy in sexual reproduction: a plant perspective. New Phytologist 226, 679-689.
22.Guolan Liu, Weiyuan Yang, Xiaojing Zhang, Tao Peng, Yi Zou, Tao Yang, Hao Wang, Xuncheng Liu, Li-zhen Tao* (2019) Cystathionine beta-lyase is crucial for embryo patterning and the maintenance of root stem cell niche in Arabidopsis. Plant Journal 99, 536-555.
2018:
21.Hao Wang#, Qiong Zhao# (co-first author), Jiaxin Fu, Xiangfeng Wang and *Liwen Jiang (2018) Re-assessment of biolistic transient expression: an efficient and robustmethod for protein localization studies in seedling-lethal mutant and juvenile plants. Plant Science 274, 2-7.
This article is highlighted as 2019 journal cover of Plant Science.
20.Xiaomin Peng, Shanshan Li and Hao Wang* (2018) Time bomb for pollen tubes: peptide RALF-1 mediated signaling. Molecular Plant 11, 518-520.
19.Xiaomin Peng#, Guitao Zhong# (co-first author) and Hao Wang* (2018) Co-expression of multiple chimeric fluorescent fusion proteins in an efficient way in plants. Journal of Visualized Experiments, DOI:10.3791/57345.
2017:
18.Guitao Zhong, Qinlong Zhu, Yingxin Li, Yaoguang Liu and Hao Wang* (2017) Once for all: a novel robust system for co-expression of multiple chimeric fluorescent fusion proteins in plants. Frontiers in Plant Science, DOI: 10.3389/fpls.2017.01071.
17.Guitao Zhong#, Ronghe Liu#, Menglong Zhuang and Hao Wang* (2017) Transient Expression of Chimeric Fluorescent Reporter Proteins in Pollen Tubes to Study Protein Polar Secretion and Dynamics. Methods in Molecular Biology, 1662, 115-124.
16.Hao Wang* and Liwen Jiang* (2017) Polar protein exocytosis: lessons from plant pollen tube. In: Obermeyer G, Feijó J (eds) Pollen Tube Tip Growth: From Biophysical Aspects to Systems Biology, Springer Nature.
15.Huei-Jing Wang Wang, Ya-Wen Hsu, Cian-Ling Guo, Wann-Nang Jane, Hao Wang, Liwen Jiang and Guang-Yuh Jauh* (2016) VPS36-Mediated MVB Formation is Critical for Plasmamembrane Protein Turnover and Vacuolar Biogenesis. Plant Physiology 173, 566-581.
2016:
14.Hao Wang, Xiaohong Zhuang, Xiangfeng Wang, Angus Ho Yin Law, Teng Zhao, Shengwang Du, Michael M. T. Loy and Liwen Jiang (2016) A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation. Plant Physiology 172, 1003-1018.
13.Teng Zhao, Sze Cheung Lau, Ying Wang, Yumian Su, Hao Wang, Aifang Cheng, Shengwang Du & M. M. T. Loy* (2016) Multicolor 4D Fluorescence Microscopy using Ultrathin Slit Bessel Light-sheets. Scientific Reports DOI: 10.1038/srep26159.
12.Hao Wang* (2016) Visualizing Plant Cells in A Brand New Way. Molecular Plant 9, 633-635.
Ph.D. and Postdoctoral Work:
11.Xiangfeng Wang#, Yi, Cai# (co-first author), Hao Wang, Yonglun Zheng, Xiaohong Zhuang, Baiying Li, and Liwen Jiang*. (2014) Trans-Golgi Network-Located AP1 Gamma Adaptins Mediate Dileucine Motif-Directed Vacuolar Targeting in Arabidopsis. The Plant Cell 26, 4102-4118.
10.Songshan Li, Qinghai He, Hao Wang, Xuming Tang, Kam Wing Ho, Xin Gao, Qian Zhang, Yang Shen, Annie Cheung, Francis Wong, Yung Hou Wong, Nancy Y. Ip, Liwen Jiang , Wing Hou Yung, Kai Liu*. (2014) Injured adult retinal axons with Pten and Socs3 co-deletion reform active synapses with suprachiasmatic neurons. Neurobiology of Disease 73, 366-376.
09. Hao Wang* and Liwen Jiang*. (2014) Immunofluorescence Labeling of Pollen Tubes. Bio-protocol 4(10): http://www.bio-protocol.org/e1131.
08.Xiaohong Zhuang#, Hao Wang#(co-first author), Sheung Kwan Lam, Caiji Gao, Xiangfeng Wang, Yi Cai and Liwen Jiang* (2013) A BAR-domain Protein SH3P2, binding to PI3P and ATG8, reveals and regulates the autophagosome formation in Arabidopsis thaliana. The Plant Cell 25, 4596-4615.
07.Hao Wang, Xiaohong Zhuang, Yi Cai, Alice Y. Cheung and Liwen Jiang* (2013) Apical F-Actin Regulated Exocytic Targeting of NtPPME1 is Essential for Pollen Tube Cell Wall Construction and Rigidity. Plant Journal 76, 367-379.
06. Liang-Zi Zhou, Sha Li, Qiang-Nan Feng, Yu-Ling Zhang ,Xinying Zhao, Yong-lun Zeng, Hao Wang, Liwen Jiang, and Yan Zhang* (2013) Protein S-ACYL Trasnferase10 Is Critical for Development and Salt Tolerance in Arabidopsis. The Plant Cell 25, 1093-1107.
05.Yi Cai, Xiaohong Zhuang, Junqi Wang, Hao Wang, Sheung Kwan Lam, Caiji Gao, Xiangfeng Wang and Liwen Jiang* (2012) Vacuolar Degradation of Two Integral Plasma Membrane Proteins, AtLRR84A and OsSCAMP1, Is Cargo Ubiquitination-Independent and Prevacuolar Compartment-Mediated in Plant Cells. Traffic 13, 1023-1040.
04.Hao Wang and Liwen Jiang* (2011) Transient Expression and Analysis of Fluorescent Reporter Proteins in Plant Pollens. Nature Protocols 6, 419-426.
03.Hao Wang, Xiaohong Zhuang, Stefan Hillmer, David G Robinson and Liwen Jiang* (2011) Vacuolar Sorting Receptor (VSR) Proteins Reach the Plasma Membrane in Germinating Pollen Tubes. Molecular Plant 4, 845-853.
02.Hao Wang, John C Rogers and Liwen Jiang* (2011) Plant RMR proteins: Unique Vacuolar Sorting Receptors that Couple Ligand Sorting with Membrane Internalization. FEBS Journal 278, 59-68.
01.Hao Wang, Yu Chung Tse, Angus Ho Yin Law, Samuel Sai-ming Sun, Yongbin Sun, Zeng-fu Xu, Stefan Hillmer, David G Robinson and Liwen Jiang* (2010) Vacuolar Sorting Receptor (VSR) and Secretory Carrier Membrane Protein (SCAMP) are Essential for Pollen Tube Growth. Plant Journal 61, 826-838.
Grants:
1. 2024-2025, the Major Research Plan of the National Natural Science Foundation of China. (Co-PI)
国家自然科学基金重大研究计划（集成项目），共同主持。
2. 2023-2026, National Natural Science Foundation of China (NSFC). (PI)
国家自然科学基金面上项目，主持。
3. 2020-2023, the Major Research Plan of the National Natural Science Foundation of China. (PI)
国家自然科学基金重大研究计划（培育项目），主持。
4. 2021-2023, Natural Science Foundation of Guangdong Province, China. (PI)
广东省自然科学基金面上项目，主持。
5. 2018-2021, National Natural Science Foundation of China (NSFC).(PI)
国家自然科学基金面上项目，主持。
6. 2017-2019, Natural Science Foundation of Guangzhou, China. (PI)
广州市科技计划项目，主持。
7. 2016-2018, Natural Science Foundation of Guangdong Province, China. (PI)
广东省自然科学基金面上项目，主持。
8. 2016-2019, National Natural Science Foundation of China (NSFC). (PI)
国家自然科学基金面上项目，主持。
Professional Activities:
Review Editor on Board: Frontiers in Plant Science, Frontiers in Cell and Developmental Biology.
学术委员会委员：中国电子显微镜学会共聚焦专业委员会，中国遗传学会细胞遗传分会。
Member: American Society of Plant Biologiests, International Congress on Sexual Plant Reproduction.
Teaching：
1. Cell Biology (Undergradaute Course)
2. Advanced Cell Biology (Postgradaute Course)
Awards Received by Graduate Students:
1. 2023 SCAU Postgraduate Oversea Research Scholarship: WENG Xun
2023 年华南农业大学研究生短期海外访学奖学金：翁洵
2. 2022 SCAU Excellent Ph.D. Thesis: YAN He
2022年华南农业大学优秀博士毕业论文：闫河
3. 2017 SCAU Postgraduate Oversea Research Scholarship: LIU Jing
2017年华南农业大学研究生短期海外访学奖学金：刘晶
4. 2017 National Postgraduate Scholarship (1st class): ZHONG Guitao
2017年国家一等奖学金：钟桂涛
5. 2016 SCAU Postgraduate Oversea Research Scholarship: ZHONG Guitao
2016年华南农业大学研究生短期海外访学奖学金：钟桂涛
