李发强

教育经历

2001/09-2008/02，美国纽约城市大学研究生院与大学中心（CUNY-Graduate School and University Center），哲学博士，生物化学专业

1998/09-2001/07，中山大学生命科学学院，理学硕士，遗传学专业

1994/09-1998/07，中山大学生命科学学院，理学学士，微生物学专业

科研与学术工作经历

2015/10至今，华南农业大学，生命科学学院，教授；

2014/01-2015/09，美国威斯康星大学麦迪逊分校，遗传系，助理研究员；

2009/01-2013/12，美国威斯康星大学麦迪逊分校，遗传系，博士后；

研究领域

1. 植物细胞自噬功能和调控机理的解析；

2. 基于自噬途径的植物靶向降解技术的开发；

3. 植物囊泡运输介导的作物重要农艺性状。

科研项目

1.国家自然科学基金面上项目，拟南芥RABG3与ATG8互作调控自噬体与液泡融合的分子机制研究，2024/01-2027/12，在研，主持。

2.国家自然科学基金面上项目，拟南芥ABA受体通过选择性细胞自噬降解的分子机制研究，2020/01-2023/12，结题，主持。

3.国家自然科学基金面上项目，细胞自噬在玉米胚乳发育过程中的作用及调控机制的研究，2018/01-2021/12，结题，主持。

4.广东省自然科学基金项目，拟南芥抗真菌毒素脱氧雪腐镰刀菌烯醇相关基因的鉴定与功能分析，2021/01-2023/12，结题，主持。

5.中山大学有害生物控制与资源利用国家重点实验室开放课题，2021/01-2023/12，结题，主持。

6.广东省自然科学基金项目，ATG8互作蛋白FYVE2a/b调控植物细胞自噬的分子机制研究，2018/05-2021/04，结题，参加。

7.华南农业大学丁颖人才重点培养对象项目，2016始，结题，主持。

8.华南农业大学高层次引进人才科研启动项目，2015/10始，结题，主持。

发表论文

1.Dong K, Deng G, Liu Y, Chen KE, Wei H, Huang X, Huang W, Zheng P, Ueda T, Vierstra RD, Huang X*, Li F*.(2025). Comprehensive genetic analyses of Arabidopsisautophagy-related 8 family reveal redundant regulatory roles during autophagy. New Phytol. 248(1):282-304.

2.Zheng X, Ma J, Li J, Chen S, Luo J, Wu J, Zhang K, Peng C, Zeng Y, Li F, Kang BH, Gao C*, Zhou J*. (2025). ATG8ylation-mediated tonoplast invagination mitigates vacuole damage. Nat Commun. 16(1):6621.

3.Shi Y, Wu Y, Li M, Luo N, Li F, Zeng S, Wang Y, Yang C*. (2025). Genome-wide identification and analysis of autophagy-related (ATG) genes in Lycium ruthenicumMurray reveals their crucial roles in salt stress tolerance. Plant Sci. 352:112371.

4.Liao Y, Li X, Ma W, Lin X, Kuang J, Zheng X, Li Z, Qiao F, Liu C, Zhou J, Li F, Li R, Kang BH, Li H, Gao C*. (2025). The plant retromer components SNXs bind to ATG8 and CLASP to mediate autophagosome movement along microtubules. Mol Plant. 18(3):416-436.

5.Zheng C, Li H, Liu Y, Huang X, Han J, Luo N, Li F*. (2025). A DUF630 and 632 domains-containing protein, ZmNRL1, acts as a positive regulator of nitrogen stress response in maize. J Exp Bot. 76(14):3984-4002.

6.Dong R, Wang W, Luo N, Li H, Liu J, Wang Y, Ye Y, Zhu H, Li F, Yu H, Cao Y*. (2025). MtNAD1 associates with the autophagy complex to contribute to the degradation of immunity-related proteins in Medicago truncatulanodules. New Phytol. 245(5):2186-2201.

7.Jiao Z, Zhan S, Liu C, Zhang H, Huang W, Li F, Ge L*. (2025). Phototropin Mediates the Accurate Movement of Trifoliate Leaves in Medicago truncatula. Plant Cell Environ. 48(10):7477-7489.

8.Huang X, Wang J, Chen S, Liu S, Li Z, Wang Z, Chen B, Zhang C, Zhang Y, Wu J, Yang X, Xie Q, Li F, An H, Huang J, Li H, Liu C, Wu X, Liu DX, Yang X, Zhou G, Zhang T*. (2024). Rhabdovirus encoded glycoprotein induces and harnesses host antiviral autophagy for maintaining its compatible infection. Autophagy. 20(2):275-294.

9.Qi H, Wang Y, Bao Y, Bassham DC, Chen L, Chen QF, Hou S, Hwang I, Huang L, Lai Z, Li F, Liu Y, Qiu R, Wang H, Wang P, Xie Q, Zeng Y, Zhuang X, Gao C, Jiang L, Xiao S*. (2023). Studying plant autophagy: challenges and recommended methodologies. Adv Biotechnol (Singap). 1(4):2.

10.Zhou J, Ma J, Yang C, Zhu X, Li J, Zheng X, Li X, Chen S, Feng L, Wang P, Ho MI, Ma W, Liao J, Li F, Wang C, Zhuang X, Jiang L, Kang BH, Gao C*. (2023). A non-canonical role of ATG8 in Golgi recovery from heat stress in plants. Nat Plants. 9(5):749-765.

11.Barros JAS, Chatt EC, Augustine RC, McLoughlin F, Li F, Otegui MS, Vierstra RD*. (2023). Autophagy during maize endosperm development dampens oxidative stress and promotes mitochondrial clearance. Plant Physiol. 193(2):1395-1415.

12.Luo N, Shang D, Tang Z, Mai J, Huang X, Tao LZ, Liu L, Gao C, Qian Y, Xie Q*, Li F*. (2022). Engineered AIM-based selective autophagy to degrade proteins and organelles in planta. New Phytol. 237(2):684-697 (*Co-corresponding author).

13.黄晓, 邓桂玲, 李发强. 基于细胞自噬的靶向降解技术及在植物研究中的应用前景. (2022).华南农业大学学报, 43(6):97-106

14.Mai J, Shang D, Li F. and Luo N*. (2022). Colocalization assay with fluorescent-tagged ATG8 using a Nicotiana benthamiana-based transient system. Bio-protocol12(16): e4486.

15.Zheng C, Yu Y, Deng G, Li H, Li F*. (2022). Network and evolutionary analysis reveals candidate genes of membrane trafficking involved in maize seed development and immune response. Front Plant Sci. 4, 13:883961.

16.Bao Y*, Gao C*, Avin-Wittenberg T*, Zhou J*, Li F*. (2022). Editorial: molecular perspectives for plant autophagy regulation. Front Plant Sci. 13, 967916 (*Co-corresponding author).

17.Zheng P, Zheng C, Otegui MS*, and Li F*. (2022). Endomembrane mediated trafficking of seed storage proteins: from Arabidopsisto cereal crops. J. Exp. Bot. 73, 1312-1326 (*Co-corresponding author).

18.Kim JH, Lee HN, Huang X, Jung H, Otegui MS, Li F*, and Chung T*. (2022). FYVE2, a phosphatidylinositol 3-phosphate effector, interacts with the COPII machinery to control autophagosome formation in Arabidopsis. Plant Cell. 34, 351-373 (*Co-corresponding author).

19.Xiao Z, Yang C, Liu C, Yang L, Yang S, Zhou J, Li F, Jiang L, Xiao S, Gao C.*, Shen W. J.* (2020). SINAT E3 ligases regulate the stability of the ESCRT component FREE1 in response to iron deficiency in plants. Integr Plant Biol. 62:1399-1417.

20.Yang C, Luo M, Zhuang X, Li F,Gao C* (2020). Transcriptional and epigenetic regulation of autophagy in plants. Trends Genet. 36:676-688.

21.McLoughlin F, Marshall RS, Ding X, Chatt EC, Kirkpatrick LD, Augustine RC, Li F, Otegui MS, Vierstra RD* (2020). Autophagy plays prominent roles in amino acid, nucleotide, and carbohydrate metabolism during fixed-carbon starvation in Maize. Plant Cell. 32:2699-2724.

22.Yang C, Shen W, Yang L, Sun Y, Li X, Lai M, Wei J, Wang C, Xu Y, Li F, Liang S, Yang C, Zhong S, Luo M, Gao C.* (2020). HY5-HDA9 module transcriptionally regulates plant autophagy in response to light-to-dark conversion and nitrogen starvation. Mol Plant. 13:515-531.

23.Liu F, Hu W, Li, F, Marshall, RS., Zarza, X, Munnik, T, & Vierstra, R. D. (2020). AUTOPHAGY-RELATED14 and its associated phosphatidylinositol 3-Kinase complex promote autophagy in Arabidopsis. Plant Cell, 32(12), 3939–3960.

24.Huang X, Zheng C, Liu F, Yang C, Zheng P, Lu X, Tian J, Chung T, Otegui MS, Xiao S, Gao C, Vierstra RD*, and Li F* (2019). Genetic analyses of the ArabidopsisATG1 kinase complex reveal both kinase-dependent and independent autophagic routes during fixed-carbon starvation. Plant Cell. 31(12): 2973-2995. (*Co-corresponding author).

25.Gou W, Li X, Guo S, Liu Y, Li F, and Xie Q* (2019). Autophagy in plant: a new orchestrator in the regulation of the phytohormones homeostasis. Int. J. Mol. Sci.20(12), 2900.

26.Liu F, Marshall RS, and Li F* (2018). Understanding and exploiting the roles of autophagy in plants through multi-omics approaches. Plant Sci. 274, 146-152.

27.McLoughlin F, Augustine RC, Marshall RS, Li F, Kirkpatrick, L.D., Otegui, MS, and Vierstra, RD* (2018). Maize multi-omics reveal roles for autophagic recycling in proteome remodelling and lipid turnover. Nat. Plants4, 1056-1070.

28.Qi H, Xia FN, Xie LJ, Yu LJ, Chen QF, Zhuang XH, Wang Q, Li F, Jiang L, Xie Q, and Xiao S* (2017). TRAF-family proteins regulate autophagy dynamics by modulating AUTOPHAGY PROTEIN6 stability in Arabidopsis. Plant Cell. 29:890-911.

29.Chen L, Li F*, and Xiao S* (2017). Analysis of plant autophagy. Methods Mol Biol1662, 267-280. (*Co-corresponding author)

30.黄晓, 李发强*. (2016). 细胞自噬在植物细胞程序性死亡中的作用. 植物学报51, 859-862.

31.Klionsky DJ*, and multiple authors including Li F, (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3^rdedition). Autophagy12, 1-222.

32.Li F, Chung T, Pennington JG, Federico ML, Kaeppler HF, Kaeppler SM, Otegui MS, and Vierstra RD* (2015). Autophagic recycling plays a central role in maize nitrogen remobilization. Plant Cell27, 1389-1408.

33.Marshall RS, Li F, Gemperline DC, Book AJ, and Vierstra RD* (2015). Autophagic degradation of the 26S proteasome is mediated by the dual ATG8/Ubiquitin receptor RPN10 in Arabidopsis. Mol. Cell58, 1053-1066.

34.Spitzer C, Li F, Buono R, Roschzttardtz H, Chung T, Zhang M, Osteryoung KW, Vierstra RD, and Otegui MS* (2015). The endosomal protein CHARGED MULTIVESICULAR BODY PROTEIN1 regulates the autophagic turnover of plastids in Arabidopsis. Plant Cell. 27:391-402

35.Li F, Chung T, and Vierstra RD* (2014). AUTOPHAGY-RELATED11 plays a critical role in general autophagy- and senescence-induced mitophagy in Arabidopsis. Plant Cell26, 788-807.

36.Li F, and Vierstra RD* (2014). ArabidopsisATG11, a scaffold that links the ATG1-ATG13 kinase complex to general autophagy and selective mitophagy. Autophagy10, 1466-1467.

37.Klionsky DJ*, and multiple authors including Li F, (2012). Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy8, 445-544.

38.Li F, and Vierstra RD* (2012a). Autophagy: a multifaceted intracellular system for bulk and selective recycling. Trends Plant Sci. 17, 526-537.

39.Li F, and Vierstra RD* (2012b). Regulator and substrate: dual roles for the ATG1-ATG13 kinase complex during autophagic recycling in Arabidopsis. Autophagy8, 982-984.

40.Suttangkakul A^#, Li F^#, Chung T, and Vierstra RD* (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell23, 3761-3779. (^#Co-first author)

41.Chen Y^#, Li F^#, and Wurtzel ET* (2010). Isolation and characterization of the Z-ISO gene encoding a missing component of carotenoid biosynthesis in plants. Plant Physiol. 153, 66-79. (^#Co-first author)

42.Li F, Tsfadia O, and Wurtzel ET* (2009). The phytoene synthase gene family in the Grasses: subfunctionalization provides tissue-specific control of carotenogenesis. Plant Signal Behav. 4, 208-211.

43.Li F, Vallabhaneni R, Yu J, Rocheford T, and Wurtzel ET* (2008b). The maize phytoene synthase gene family: overlapping roles for carotenogenesis in endosperm, photomorphogenesis, and thermal stress tolerance. Plant Physiol. 147, 1334-1346.

44.Li F, Vallabhaneni R, and Wurtzel ET* (2008a). PSY3, a new member of the phytoene synthase gene family conserved in the Poaceae and regulator of abiotic stress-induced root carotenogenesis. Plant Physiol. 146, 1333-1345.

45.Li F, Murillo C, and Wurtzel ET* (2007). Maize Y9 encodes a product essential for 15-cis-zeta-carotene isomerization. Plant Physiol. 144, 1181-1189.

46.Gallagher CE, Matthews PD, Li F, and Wurtzel ET* (2004). Gene duplication in the carotenoid biosynthetic pathway preceded evolution of the grasses. Plant Physiol. 135, 1776-1783.

教学活动

1. 承担《分子生物学》、《细胞生物学》等本科课程。

2. 指导本科生获得2021年iGEM全球总决赛金奖和2024年全国大学生生命科学竞赛一等奖。

（2026年4月更新）